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. 2016 Jan 20;7:10357. doi: 10.1038/ncomms10357

Figure 3. Monitoring Hsp33-client-binding interactions using site-specific 19F NMR.

Figure 3

19F NMR spectra of select Hsp33M172StFPA variants in the absence (blue) or presence (magenta) of (a) NPY or (b) NPY labelled with the paramagnetic spin-label TEMPO. The incubation temperature was set to 35 °C. All experiments were conducted at least three times.