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. 2016 Jan 20;7:10357. doi: 10.1038/ncomms10357

Table 1. Summary of identified Hsp33ox-NPY crosslinked peptides.

Crosslinker Protease Modified amino acid* Hsp33 peptide sequence
ABAS (7 Å) PK 44 E35NHDYPQPVKN45
  PK 62 L61KFDGD66
  PK 62 L61KFDGDIT68
  PK 107 G98EIPENADLK107
  PK 198 T194ETIK198
CBDPS (14 Å) T 1 M1IMPQHDQLHR11
  T 12 Y12LFENFAVR20
  PK 62 L61KFD64
  PK 107 G98EIPENADLK107
  PK 166 G165KPA168
  PK 231 D227VEFK231
EDC (0 Å) T 102 V96QGEIPENADLK107
  T 150 S149EQLPTR155
  PK 198 T196IKTEELLTLPANEV210

ABAS, azido-benzoic-acid-succinimide; CBDPS, cyanur-biotin-dimercapto-propionyl-succinimide; EDC, (3-dimethylaminopropyl)carbo diimide; NPY, neuropeptide Y; PK, protease K; T, trypsin.

*Bold indicates residue was identified by at least two different crosslinkers. Bold and italicized alphabets indicate amino acids in Hsp33 peptides that were found crosslinked to NPY.