Table 1. Summary of identified Hsp33ox-NPY crosslinked peptides.
Crosslinker | Protease | Modified amino acid* | Hsp33 peptide sequence |
---|---|---|---|
ABAS (7 Å) | PK | 44 | E35NHDYPQPVKN45 |
PK | 62 | L61KFDGD66 | |
PK | 62 | L61KFDGDIT68 | |
PK | 107 | G98EIPENADLK107 | |
PK | 198 | T194ETIK198 | |
CBDPS (14 Å) | T | 1 | M1IMPQHDQLHR11 |
T | 12 | Y12LFENFAVR20 | |
PK | 62 | L61KFD64 | |
PK | 107 | G98EIPENADLK107 | |
PK | 166 | G165KPA168 | |
PK | 231 | D227VEFK231 | |
EDC (0 Å) | T | 102 | V96QGEIPENADLK107 |
T | 150 | S149EQLPTR155 | |
PK | 198 | T196IKTEELLTLPANEV210 |
ABAS, azido-benzoic-acid-succinimide; CBDPS, cyanur-biotin-dimercapto-propionyl-succinimide; EDC, (3-dimethylaminopropyl)carbo diimide; NPY, neuropeptide Y; PK, protease K; T, trypsin.
*Bold indicates residue was identified by at least two different crosslinkers. Bold and italicized alphabets indicate amino acids in Hsp33 peptides that were found crosslinked to NPY.