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A, Schematic diagram indicates binding sites of the primers used for colony PCR; B, colony PCR to check transformed Agrobacteria. Lane 1: colony PCR using Kozak FOR and NOS R primers; lane 2: colony PCR using Kozak FOR and R-Core primers; lane M: 100 bp DNA ladder; lane C-: untransformed-Agrobacterium (without construct) as negative control. Lane C+: construct as positive control.
