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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: Dev Cell. 2016 Jan 11;36(1):24–35. doi: 10.1016/j.devcel.2015.12.013

Figure 4. Formation of medial apical actin of MCCs highly correlates with expansion dynamics of the apical domain.

Figure 4

(A) Segmentation schematic of the cortical (green) and the medial (red) region of a MCC within the plane of the exposed apical area. (B) Representative image sequence of automatically segmented cortical and medial region within apically expanding MCC (visualized by α-tubulin UtrCH-GFP). (C) Representative - effective actin (ratio of mean medial to mean cortical actin) dynamics (red) during the apical area (black) expansion process. Crosses, data points; line, data smoothed by factor 5. (D) Effective apical actin concentration for the consecutive apical domain sizes, categorized by binned area, in control cells, n=9 cells from 9 embryos. Data represent mean and variance. (E) Effective apical actin concentration as a function of apical area in control cells, n=9 cells from 9 embryos. Scale bar, 10µm. a.u., arbitrary units.