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. 2016 Feb 1;17:87. doi: 10.1186/s12864-016-2394-y

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© Martínez et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Infectivity of TEV recombinant clones with tagged NIa. a and b N. benthamiana plants non-inoculated (mock) and agroinoculated with wild-type TEV (TEV-wt) and TEV clones with TST-NIa at five different positions (TEV-TSTNIa1 to TEV-TSTNIa5). Pictures of representative plants were taken at 9 (a) or 26 (b) days postinoculation (dpi). c RT-PCR analysis of the viral progenies from plants infected with TEV-wt (lanes 2 to 4, 9 to 11 and 16 to 18), TEV-TSTNIa1 (lanes 5 to 7), TEV-TSTNIa2 (lanes 12 to 14) and TEV-TSTNIa5 (lanes 19 to 21). The control RT-PCR reaction (lane 1) contained all primers and no template. Lanes 8, 15 and 22, DNA marker ladder with sizes on the right side in kbp. Red arrows point the PCR products delayed as a consequence of insertion of the TST cDNA in the viral progenies