Table 1.

The primer sequences to amplify the CDS of MyD88 gene in chicken.

Name	Amplicon size (bp)	Sequence (5′-3′)	Production (bp)	Anneal temperature (°C)	Region
P1	19	F: GGCTCCTTCCAACCCAAAC	793	59.0	exon1
	21	R: AGACCGATCTCACCTCACCAC
P2	22	F: CAAGAAGGCACTGGGTAAACTC	483	54.6	exon2
	21	R: GAATAGGCAACGGGAAGAATG
P3	20	F: TCTGTCAAAGGCTGGGGAAG	297	54.0	exon3
	19	R: ACACTGAGCTGCCCCAAGC
P4	24	F: GTTCCTGCTCAACCACAACTAAAG	443	52.5	exon4
	22	R: GGGTTCTGGTTCAGTAGGCATC
P5	23	F: TAGAAGCAGGATGTGAGTGTGGC	501	59.6	exon5
	25	R: GCAGTGACTCAGTCTTTAAGCGAAT