Table 1.

Sequence of oligonucleotides used to amplify insert (IF/IR) and vector (VF/VR) DNAs during prolonged overlap extension (POE) PCR^∗.

Primer	Sequence 5′-3′
VF	CCTTGCCCAGCCCTGATAGAAGCTTGGATCCGGAGTCGAACCATAAAAGC
VR	TGGCAGGGCCCAGGGGGGTCATATGAGCTGATGCCGAATACGTAAAGGTA
IF	TACCTTTACGTATTCGGCATCAGCTCATATGACACCTCTGGGCCCTGCCA
IR	GCTTTTATGGTTCGACTCCGGATCCAAGCTTCTATCAGGGCTGGGCAAGG

^∗Primers were designed using online available software (http://www.xiaozhouzhang.com). AAGCTT and CATATG (underlined sequences) are the recognition sites for the HindIII and NdeI restriction endonucleases, respectively.