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Purification and recovery of human GCSF expressed in B. subtilis. Culture supernatant of transformed cells, grown in 1 liter of 2x L-MAL medium for 72 hours at 30°C with OD600 6.0, was clarified by centrifugation and used for the purification of recombinant GCSF.
| Steps | TSP∗ | GCSF | Recovery | Purity |
|---|---|---|---|---|
| (mg) | (mg) | (%) | (%) | |
| Culture supernatant | 720 | 122 | 100 | 17 |
| Ammonium sulphate precipitation | 235 | 115 | 94 | 49 |
| Dialysis | 212 | 110 | 90 | 52 |
| FPLC purification (QFF) | 107 | 96 | 78 | 90 |
∗TSP: total secretory protein.
