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. 2015 Dec 31;2016:2592319. doi: 10.1155/2016/2592319

Table 1.

Antibodies conditions used in the Western blot analyses.

Gene Primary antibody Secondary antibody
Phospho-ERK1/2 Y204/Y187
(42–44 kDa)		 1 : 1000 (Cell Signaling; #4370)
4°C, O/N		 Anti-mouse,
1 : 2000, RT, 1 h
ERK1/2
(42–44 kDa)		 1 : 5000 (Sigma; M3807),
RT, 2 h		 Anti-rabbit,
1 : 5000, RT, 1 h
Phospho-CREB S133
(43 kDa)		 1 : 1000 (Cell Signaling; #4276),
4°C, O/N		 Anti-rabbit,
1 : 2000, RT, 1 h
CREB
(43 kDa)		 1 : 1000 (Cell Signaling; #9197),
4°C, O/N		 Anti-rabbit,
1 : 2000, RT, 1 h
Phospho-AKT S473
(60 kDa)		 1 : 1000 (Cell Signaling; #4060),
4°C, O/N		 Anti-rabbit,
1 : 2000, RT, 1 h
AKT
(60 kDa)		 1 : 1000 (Cell Signaling; #9272),
RT, 2 h		 Anti-rabbit,
1 : 1000, RT, 1 h
Phospho-GSK3β S9
(46 kDa)		 1 : 1000 (Cell Signaling; #9336),
4°C, O/N		 Anti-rabbit,
1 : 5000, RT, 1 h
GSK3β
(46 kDa)		 1 : 2500 (BD Transduction; 610201)
RT, 2 h		 Anti-mouse,
1 : 5000, RT, 1 h
β-ACTIN 1 : 10000 (Sigma; A5441),
RT, 1 h		 Anti-mouse,
1 : 10000, RT, 1 h