Fig. 3.

Msx1 and Meox2 proteins interact in vivo with the 145-bp Myf5 regulatory sequence. ChIP experiments were carried out with two different anti-HA antibodies (aHA1-2), to immunoprecipitate chromatin prepared from the thoracic region of the trunk including forelimb buds (forelimb region), of E10-E10.5 Msx1^Tag/Tag embryos, or with anti-Meox2 antibodies to immunoprecipitate chromatin prepared from the equivalent region of wild-type embryos at E10.5. Histograms represent the fold change in occupancy of the 145-bp Myf5 element, versus two different negative control regions located respectively at −257.5 kb (black) and −55.2 kb (grey) upstream of the Myf5 gene transcription start site (Bajard et al., 2006; Giordani et al., 2007). A control using IgGs from non-immune serum is shown for experiments with wild-type chromatin. Results on the right part of the figure were obtained with ChIP on extracts from interlimb regions, excluding limb buds, of Msx1^Tag/Tag embryos at E10.5, using anti-HA antibodies or control non immune IgGs. Biological replicates of these ChIP experiments were carried out with three different preparations of chromatin. Error bars indicate s.e.m.