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Synthesis of PEGx-APV-O-acetyl and PEGx-APV-OH nanocarrier conjugates. Reagents and conditions: (i) DMF, DIPEA, acetic anhydride, room temperature, 12 h; (ii) DMF, DIPEA, mPEGx-NHS (x = 2, 5, 10, 30 kDa), room temperature, 24 h; and (iii) 0.1 N HCl, neutralized with NaHCO3.
