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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Drug Deliv Transl Res. 2016 Feb;6(1):1–16. doi: 10.1007/s13346-015-0269-4

Scheme 2.

Scheme 2

Synthesis of fluorescein-labeled APV-PEG (APF) and Bac7 CPP-PEG NCs (BPF): (A) APV-PEG3.4kDa-FITC; (B) Structure of Bac7 analog; and (C) Bac7 CPP-PEG3.4kDa-FITC. Reagents and conditions: (i) DMF containing EDCI and HOAt, room temperature, 16 h; and (ii) DMF, room temperature, 4 h. PEG-fluorescein is linked to APV via an amide bond and to Bac7 via a thioether bond.