Table 3.
Strain or plasmid | Relevant characteristicsa | Source or referenceb |
---|---|---|
Bacterial strains | ||
E. coli | ||
TOP10 | Invitrogen | |
ER2275 | RecA− McrBC− | NEB |
C. acetobutylicum | ||
ATCC824 | Wild type | ATCC |
MGCΔcac1502 | ΔCA_C1502 | This study |
MGCΔcac1502Δupp | ΔCA_C 1502Δ CA_C 2879 | This study |
MGCΔcac1502ΔuppΔcac3535 | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535 | This study |
MGCΔcac1502ΔuppΔcac3535ΔctfAB | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_P0162-3 | This study |
MGCΔcac1502ΔuppΔcac3535ΔldhA | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_C 0267 | This study |
Plasmids | ||
pAN1 | Cmr, φ3TI, p15A origin | [27] |
pKD4 | Apr Kmr | [18] |
pETSPO | Cmr MLSr | [4] |
pUC18 | Apr | Fermentas |
pUC18-FRT-MLS2 | Apr MLSr | This study |
pCons2-1 | Cmr | This study |
pCR-BluntII-TOPO | Zeor Kmr | Invitrogen |
pCIP2-1 | Cmr | This study |
pREPcac15 | Cmr MLSr ΔCA_C1502 | This study |
pCIPcac15 | Cmr MLSr ΔCA_C1502 | This study |
pREPupp | Cmr MLSr Δupp | This study |
pCP20 | Apr Cmr FLP | [29] |
pSOS95 | Apr MLSr, acetone operon, repL gene, ColE1 origin | [32] |
pCLF1 | Cmr FLP | This study |
pCR4-TOPO-Blunt | Apr Kmr | Invitrogen |
pCons::upp | Cmr MLSr upp | This study |
pREPCAC3535::upp | Cmr MLSr upp ΔCA_C3535 | This study |
pREPctfAB::upp | Cmr MLSr upp ΔctfAB | This study |
pREPldhA::upp | Cmr MLSr upp ΔldhA | This study |
a RecA − homologous recombination abolished, McrBC − lacking methylcytosine-specific restriction system, Cm r chloramphenicol resistance, Ap r ampicillin resistance, MLS r macrolide lincosamide and streptogramin B resistance, Zeo r zeomycin resistance, φ3TI φ3TI methyltransferase, repL Gram-positive origin of replication from pIM13
b NEB New England BioLabs, ATCC American Type Culture Collection (Rockville, MD)