Table 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or referenceb |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| TOP10 | Invitrogen | |
| ER2275 | RecA− McrBC− | NEB |
| C. acetobutylicum | ||
| ATCC824 | Wild type | ATCC |
| MGCΔcac1502 | ΔCA_C1502 | This study |
| MGCΔcac1502Δupp | ΔCA_C 1502Δ CA_C 2879 | This study |
| MGCΔcac1502ΔuppΔcac3535 | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535 | This study |
| MGCΔcac1502ΔuppΔcac3535ΔctfAB | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_P0162-3 | This study |
| MGCΔcac1502ΔuppΔcac3535ΔldhA | ΔCA_C 1502ΔCA_C 2879ΔCA_C 3535ΔCA_C 0267 | This study |
| Plasmids | ||
| pAN1 | Cmr, φ3TI, p15A origin | [27] |
| pKD4 | Apr Kmr | [18] |
| pETSPO | Cmr MLSr | [4] |
| pUC18 | Apr | Fermentas |
| pUC18-FRT-MLS2 | Apr MLSr | This study |
| pCons2-1 | Cmr | This study |
| pCR-BluntII-TOPO | Zeor Kmr | Invitrogen |
| pCIP2-1 | Cmr | This study |
| pREPcac15 | Cmr MLSr ΔCA_C1502 | This study |
| pCIPcac15 | Cmr MLSr ΔCA_C1502 | This study |
| pREPupp | Cmr MLSr Δupp | This study |
| pCP20 | Apr Cmr FLP | [29] |
| pSOS95 | Apr MLSr, acetone operon, repL gene, ColE1 origin | [32] |
| pCLF1 | Cmr FLP | This study |
| pCR4-TOPO-Blunt | Apr Kmr | Invitrogen |
| pCons::upp | Cmr MLSr upp | This study |
| pREPCAC3535::upp | Cmr MLSr upp ΔCA_C3535 | This study |
| pREPctfAB::upp | Cmr MLSr upp ΔctfAB | This study |
| pREPldhA::upp | Cmr MLSr upp ΔldhA | This study |
a RecA − homologous recombination abolished, McrBC − lacking methylcytosine-specific restriction system, Cm r chloramphenicol resistance, Ap r ampicillin resistance, MLS r macrolide lincosamide and streptogramin B resistance, Zeo r zeomycin resistance, φ3TI φ3TI methyltransferase, repL Gram-positive origin of replication from pIM13
b NEB New England BioLabs, ATCC American Type Culture Collection (Rockville, MD)