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. 2016 Feb 1;13:24. doi: 10.1186/s12974-016-0491-0

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© Lee et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — RGS10-null CD4+ T cells displayed attenuated migration to CXCL12, a ligand for CXCR4. CD4+ T cell migration to CXCL12 was tested in an in vitro transwell chemotaxis assay. a Histogram shows the levels of chemokine receptor CXCR4 staining on CD3+ CD19− CD4+ T lymphocytes from WT and RGS10-null mouse spleens, as determined by flow cytometry. b Percentage of CD4+ T cells from WT or RGS10 null mice (n = 3) that migrated to the lower chamber containing serum free-medium (VEH), CXCL12 (10 nM) or under control conditions (CXCL12 in both lower and upper chambers) *, p < 0.05, two-way ANOVA