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Validation of proteins by Western blot analysis. (a) Lane 1 is control and Lane 2 represents sample from bilateral varicocele group. (b) β-actin was used as an in-house protein. TEKT3 and TCP11 overexpression in men with bilateral varicocele was validated by Western blot analysis. (c and d) shows the quantification of the overexpressed proteins TEKT3 and TCP11 in the bilateral varicocele group by measuring the protein band intensity.
