Table 2.
IC50/EC50 values for drug induced effects on different parameters measured in hiPSC derived hepatocytes (hiPS-HEP) and HepG2.
| Compound | Amiodarone | Doxycycline | Tetracycline | |||
|---|---|---|---|---|---|---|
| Cell line | hiPS-HEP | HepG2 | hiPS-HEP | HepG2 | hiPS-HEP | HepG2 |
| Fixed | ||||||
| Nucleus | ||||||
| Cell count | 31.0 | 7.6 | 107 | 165 | 432 | 558 |
| MI | 95.7 | 140 | 47.2 | 892 | 211 | 348 |
| Area | 2.2 | 39.5 | 96.1 | OR | 105 | 464 |
| Steatosis | ||||||
| Count | 21.0 | 19.5 | 115 | 260 | 184 | 36.3 |
| MI | 34.1 | 23.6 | 441 | OR | 418 | 361 |
| Area | 25.5 | 30.6 | — | 398 | 213 | 287 |
| Phospholipidosis | ||||||
| Count | 6.1 | 8.9 | NA | NA | NA | NA |
| MI | 6.0 | 35.6 | NA | NA | NA | NA |
| Area | 4.5 | 7.3 | NA | NA | NA | NA |
|
| ||||||
| Live | ||||||
| Nucleus | ||||||
| Cell count | 21.9 | 7.2 | 69.4 | 120.3 | 243 | 836 |
| MI | 22.4 | 25.0 | 120 | 153 | 380 | 959 |
| Area | 10.4 | 29.1 | 204.3 | — | 211 | 336 |
| MMP | ||||||
| MI | 20.8 | 345 | 644 | 622 | — | 265 |
| ROS | ||||||
| MI | 26.5 | 4.6 | 10.5 | 115 | 160 | 1149 |
| PMP | ||||||
| MI | 42.2 | 147 | 26.9 | OR | OR | OR |
Four-parameter variable slope curve fit using the least squares method was applied for generating dose response curves in GraphPad Prism software. Parameters with the lowest and second lowest IC50/EC50 values are denoted in bold; values in italics had an ambiguous near complete dose response curve fit; [OR], curves which were out of range. Values denoted are mean values from three different experimental batches (N = 3). All values are represented as μM.