Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 3;17(1):82–89. doi: 10.1002/cbic.201500454

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

A) Size‐exclusion chromatography profiles of acylated USH3 expressed under different conditions. Red curve: in Rosetta cells, in the presence of both myristic and palmitic acid (20 h, 20 °C), two elution peaks are observed. Green curve: in T7 cells, in the presence only of myristic acid (20 h, 20 °C), three elution peaks are observed. Blue curve: in T7 cells, in the presence of both myristic and palmitic acid (5 h, 28 °C), one elution peak is observed. B) and C) Mass spectrometric analysis after separate pooling of the fractions corresponding to peaks 1 and 2, respectively. D) RP‐HPLC analysis of peak 3 of the red curve in (A) after pooling of its fractions. E) and F) RP‐HPLC and MS analysis of peak 2 of the blue curve after pooling of its fractions.