Fig. 5.

Upregulation of leptin receptor signaling in the gastric mucosa of HFD-fed WT mice. a Western blot analysis of the phosphorylation of ObRb, STAT3, Akt and ERK1/2 in the gastric mucosa of WT, ob/ob and db/db mice fed CD and HFD for 1 and 4 weeks. A total of 3 mice were used in each diet group per one experiment. Each experiment was repeated twice and representative data are shown. b Immunohistochemistry and (c) western blot analysis of phosphorylation of ObR in the gastric mucosa of db/db mice and WT mice fed CD or HFD for 3 weeks. We utilized 3 mice in each analysis, and representative data are shown. Arrows indicate phosphorylated ObR-positive cells