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. 2016 Feb 1;16:35. doi: 10.1186/s12870-016-0728-1

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© Du et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Relative VHb expression in VHb maize seedlings. Total RNA samples were isolated from the leaves of transgenic Zheng58 (VHb) and CML50 (VHb) seedlings, reverse-transcribed into cDNA, and used for real-time qRT-PCR. The relative expression levels of VHb were calculated by using the maize actin1 gene (GRMZM2G126010) as the internal reference. The results represent the mean values ± SD of three independent analyses