Table 3.

Total precision (variance, standard deviation and CV %) of PCR assays for HCV genotypes 2b, 1a, 3a and 6a (mean log10 HCV RNA viral load) at different key concentrations - within assay ranges

HCV genotype	Expected HCV RNA (IU/ml)	Expected HCV RNA (log10 IU/ml)	No. tests performed	Observed mean HCV RNA (log10 IU/ml)	Difference of observed and expected HCV RNA (log10 IU/ml)	Total precision variance (SD) of log10 HCV RNA	Lognormal CV%
WWHV303-02 (1b)	25	1.398	9	1.372	−0.026	0.019 (0.137)	10.01
	50	1.699	9	1.665	−0.034	0.004 (0.067)	4.023
	100	2	9	1.951	−0.049	0.008 (0.088)	4.486
	1000	3	9	2.959	−0.041	0.022 (0.150)	5.055
	10000	4	9	4.015	0.015	0.003 (0.057)	1.410
	100000	5	9	5.049	0.049	0.002 (0.050)	0.989
WWHV303-04 (2a)	25	1.398	9	1.411	0.013	0.012 (0.109)	7.755
	50	1.699	9	1.710	0.011	0.009 (0.093)	5.453
	100	2	9	2.022	0.022	0.016 (0.126)	6.231
	1000	3	9	2.886	−0.114	0.017 (0.130)	4.513
WWHV303-11 (3a)	25	1.398	9	1.379	−0.019	0.013 (0.113)	8.181
	50	1.699	9	1.737	0.038	0.007 (0.080)	4.664
	100	2	9	2.044	0.044	0.019 (0.139)	6.798
	1000	3	9	2.893	−0.107	0.023 (0.150)	5.185
WWHV303-19 (6a)	25	1.398	9	1.413	0.015	0.013 (0.112)	7.958
	50	1.699	9	1.712	0.013	0.008 (0.087)	5.100
	100	2	9	1.980	−0.02	0.026 (0.162)	8.170
	1000	3	9	2.915	−0.085	0.017 (0.130)	4.478

Abbreviations: SD standard deviation