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. 1968 Jan;21(1):35–40. doi: 10.1136/jcp.21.1.35

Significance for the diagnosis of iron overload of histochemical and chemical iron in the liver of control subjects

A Weinfeld 1, P Lundin 1, O Lundvall 1
PMCID: PMC473659  PMID: 5697333

Abstract

Storage iron was examined in surgical liver biopsy specimens in 43 haematologically normal and otherwise healthy adult individuals. These patients had no history of unphysiological iron losses nor of unphysiological iron intake. Histochemical iron was estimated in parenchymal and Kupffer cells and graded from 0 to 4+. Stainable iron of grade 1+ or more was present in parenchymal cells in 23 of the 27 men. Six of them had a 3+ grade. In nine cases iron was also visible in Kupffer cells. Visible iron was absent in most of the menstruating women. The mean total nonhaemin iron concentration for the male group was 80·2 (19·4 to 227·0), for the postmenopausal women 50·7 (19·3 to 106·6), and for the menstruating women 23·5 (5·5 to 65·9) mg./100 g. dry weight. The mean value for the women was significantly lower than the mean value for the men. There was a significant correlation between the histochemical grades of iron and chemically determined nonhaemin iron, but the degree of overlapping was considerable.

The presence of stainable iron in the parenchymal liver cells is a normal finding. The significance of the present results with reference to familial studies of haemochromatosis is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOTHWELL T. H., COHEN I., ABRAHAMS O. L., PEROLD S. M. A familial study in idiopathic hemochromatosis. Am J Med. 1959 Nov;27:730–738. doi: 10.1016/0002-9343(59)90189-5. [DOI] [PubMed] [Google Scholar]
  2. Balcerzak S. P., Westerman M. P., Lee R. E., Doyle A. P. Idiopathic hemochromatosis. A study of three families. Am J Med. 1966 Jun;40(6):857–873. doi: 10.1016/0002-9343(66)90201-4. [DOI] [PubMed] [Google Scholar]
  3. FREY W. G., 3rd, MILNE J., JOHNSON G. B., Jr, EBAUGH F. G., Jr Management of familial hemochromatosis. N Engl J Med. 1961 Jul 6;265:7–12. doi: 10.1056/NEJM196107062650102. [DOI] [PubMed] [Google Scholar]
  4. HUTCHISON H. E. The significance of stainable iron in sternal marrow sections; its application in the control of iron therapy. Blood. 1953 Mar;8(3):236–248. [PubMed] [Google Scholar]
  5. JOHNSON G. B., Jr, FREY W. G., 3rd Familial aspects of idiopathic hemochromatosis. JAMA. 1962 Mar 10;179:747–751. doi: 10.1001/jama.1962.03050100001001. [DOI] [PubMed] [Google Scholar]
  6. KAUTZSCH E. [Transport and storage of iron in liver cirrhosis]. Med Klin. 1959 Oct 16;54:1899–1905. [PubMed] [Google Scholar]
  7. LUNDIN P., PERSSON E., WEINFELD A. COMPARISON OF HEMOSIDERIN ESTIMATION IN BONE MARROW SECTIONS AND BONE MARROW SMEARS. Acta Med Scand. 1964 Mar;175:383–390. doi: 10.1111/j.0954-6820.1964.tb00586.x. [DOI] [PubMed] [Google Scholar]
  8. MACDONALD R. A. Idiopathic hemochromatosis. A variant of portal cirrhosis and idiopathic hemosiderosis. Arch Intern Med. 1961 Apr;107:606–616. doi: 10.1001/archinte.1961.03620040132015. [DOI] [PubMed] [Google Scholar]
  9. PECHET G. S., FRENCH S. W., LEVY J., MACDONALD R. A. HISTOLOGIC AND CHEMICAL TISSUE IRON. SIGNIFICANCE FOR HEMOCHROMATOSIS. Arch Pathol. 1965 May;79:452–461. [PubMed] [Google Scholar]
  10. PECHET G. S., MACDONALD R. A. Idiopathic hemosiderosis. Relation to idiopathic hemochromatosis. N Engl J Med. 1962 Jul 5;267:6–10. doi: 10.1056/NEJM196207052670102. [DOI] [PubMed] [Google Scholar]
  11. PETERS T., GIOVANNIELLO T. J., APT L., ROSS J. F. A new method for the determination of serum iron-binding capacity. I. J Lab Clin Med. 1956 Aug;48(2):274–279. [PubMed] [Google Scholar]
  12. SCHEUER P. J., WILLIAMS R., MUIR A. R. Hepatic pathology in relatives of patients with haemochromatosis. J Pathol Bacteriol. 1962 Jul;84:53–64. [PubMed] [Google Scholar]
  13. WEINFELD A. STORAGE IRON IN MAN. Acta Med Scand Suppl. 1964;427:SUPPL 427–427:1+. [PubMed] [Google Scholar]
  14. WILLIAMS R., SCHEUER P. J., SHERLOCK S. The inheritance of idiopathic haemochromatosis. A clinical and liver biopsy study of 16 families. Q J Med. 1962 Jul;31:249–265. [PubMed] [Google Scholar]
  15. ZIMMERMAN H. J., CHOMET B., KULESH M. H., McWHORTER C. A. Hepatic hemosiderin deposits. Incidence in 558 biopsies from patients with and without intrinsic hepatic disease. Arch Intern Med. 1961 Apr;107:494–503. doi: 10.1001/archinte.1961.03620040020003. [DOI] [PubMed] [Google Scholar]

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