Figure 7. E202A expression or MAL2 knockdown does not impair albumin synthesis or Golgi delivery.

(A) E202A-expressing cells were incubated at 19°C for 0, 2 or 4 h as indicated. Cells were immunolabelled for albumin (a–c) or E202A (d–f). Arrows indicate recovered Golgi labelling of albumin in cells expressing E202A after the temperature block. (B) MAL2-knockdown cells were incubated at 19°C for 0, 2 or 4 h as indicated. Cells were immunolabelled for albumin. Asterisks indicate cells with knocked down MAL2 expression. Arrows mark recovered albumin labelling in knockdown cells after the temperature block. (C) Albumin and E202A distributions were monitored after incubation at 19°C for 4 h, then released back to 37°C for 15 or 30 min as indicated. Arrows mark E202A at the Golgi prior to redistribution back to the peripheral puncta. Scale bar, 10 μm. E202A-expressing cells were scored for the presence of albumin labelling in the Golgi after release from 19°C⁻for the indicated times. The percentage of infected cells positive for albumin labelling is plotted and shown in the top histogram. Values are expressed as the means ± S.E.M. Measurements were performed on at least three independent experiments. E202A-expressing cells were scored for the percentage of infected cells positive for albumin labelling is plotted in the bottom histogram. Values are expressed as the mean of two independent experiments.