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. 2016 Feb 1;16:51. doi: 10.1186/s12885-016-2074-y

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© Legendre et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Intracellular iron concentration is depleted by both deferasirox and irradiation in U251 cells but only under normoxic condition. Intracellular iron concentration in U251 glioblastoma cells cultivated at 21 % (a) or 3 % (b) of oxygen and in U87 glioblastoma cells cultivated at 21 % (c) or 3 % (d) of oxygen in untreated condition (CONT) or 3 days after treatment with 10 μM of deferasirox (DFX) in non-irradiated condition (0 Gy) or following irradiation with 16 Gy (16 Gy). Intracellular iron concentration is expressed in nmol per mg of protein as mean ± standard deviation (SD) (n = 3). One-way ANOVA was performed between DFX treatment and CONT in non-irradiated (0 Gy) or irradiated (16 Gy) conditions (^*, p-value ≤0.05; ^***, p-value ≤0.001). Two-way ANOVA was performed between non-irradiated (0 Gy) condition and irradiated (16 Gy) condition (^$$$, p-value ≤0.001)