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. 2016 Feb 2;35:24. doi: 10.1186/s13046-016-0302-6

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© D’Eliseo et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Ectopic GrB upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB transfection efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p < 0.01; **p < 0.0001. Representative experiments out of at least three