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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Angew Chem Int Ed Engl. 2015 Nov 13;54(52):15782–15788. doi: 10.1002/anie.201508783

Figure 3.

Figure 3

CholA-anchored glycopolymers recycle from reservoirs inside the cell to the cell surface. a) Jurkats were labeled with 5 μM AF488-capped glycopolymers. b) Jurkats loaded with CholA glycopolymers and incubated with AF647-transferrin. c) Jurkats loaded with CholA-anchored glycopolymers, then treated with anti-AF488 antibody to quench cell surface fluorescence, then allowed to incubate at the indicated temperature. d) A model for the trafficking of CholA-anchored glycopolymers. Scale bars are 10 μm.