Figure 7.

N-Acetylcysteine amide attenuates H₂O₂-induced effects on RPE-derived MPs. (A) Microparticles isolated from control and 500 μM H₂O₂-treated RPE cells with N-acetylcysteine amide (+ NACA) or without NACA (−NACA) pretreatment were stained with MFG-E8-FITC and quantified by flow cytometry. (B) Representative images taken with ×63 oil immersion lens and ×10 digital zoom. Scale bars: 2.5 μm. (C–E) Microparticles were stained for membrane complement regulatory proteins CD46 (C), CD55 (D), and CD59 (E). Data are presented as mean ± SD (n = 3–6). ***P < 0.0001 compared with controls in the absence of NACA (−NACA); #P < 0.001 compared with H₂O₂-treated groups in the absence of NACA (−NACA).