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. 2016 Jan 6;2016:8373020. doi: 10.1155/2016/8373020

Figure 3.

Figure 3

Involvement of the CB1 receptor on the neuroprotective actions of HU-210. (a) ChAT immunoreactivity. The selective inverse agonist AM251 attenuated the neuroprotective actions of HU-210 on cholinergic amacrine cells ((A) control, n = 12; (B) chemical ischemia mixture, n = 12; (C) HU-210 (10−5 M), n = 3; (D) HU-210 (10−5 M) + AM251 (10−5 M)). Arrows depict ChAT-immunoreactive amacrine cells. Scale bar: 50 μm. (b) Effect of cannabinoids on retinal cell death. TUNEL staining was prominent in the chemical ischemia incubated tissues ((B) n = 2) while less staining was observed in the control tissues ((A) n = 2). TUNEL staining substantiates the neuroprotective effects of HU-210 ((C) 10−5 M, n = 2) and the reduced neuroprotection (increased TUNEL staining) in the presence of AM251 ((D) 10−5 M, n = 3). ONL: outer nuclear layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer.