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. 2016 Jan 6;6(1):150149. doi: 10.1098/rsob.150149

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 8. — Immunoblot analysis of GlxA maturation in S. lividans TK24 and tat mutant strains grown for for 2 days on R5 medium (a) or grown for 24 h in TSBS medium (b) in the presence (+) or absence (−) of 10 µM CuSO₄. The two bands indicated with the solid arrows represent GlxA, the upper band being immature GlxA (no Tyr–Cys cross-link) and the lower band being mature GlxA (with Tyr–Cys cross-link). The band indicated with a dashed arrow indicates EF-Tu, which serves as a control for the total amount of protein loaded on the gel. Molecular weight markers are indicated in kDa.