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. 2016 Jan 20;6(1):150169. doi: 10.1098/rsob.150169

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Distribution of phosphoinositides in CdsA^ms1 male germ cells. (a,b) Confocal micrographs of the squashed and fixed preparations of elongated spermatids expressing the PI4P-binding RFP-PH-FAPP transgene. RFP-PH-FAPP localizes to punctate structures both in the wild-type (a) and CdsA^ms1 (b) elongated spermatids. (c–f) Confocal micrographs of male germ cells expressing PLCδ-PH-GFP, which binds PI(4,5)P₂. In spermatocytes (left side of images) and in elongated spermatids (right side of images), PI(4,5)P₂ is localized to the plasma membrane in wild-type (c) and CdsA^ms1 (d) testes. Images were taken with the same exposure time in the case of each transgene, with or without the CdsA^ms1 homozygotes. Membrane to cytoplasmic RFP intensity (e) or GFP intensity (f) ratios were calculated from measurements of mean pixel intensities within the same sized area of membrane versus cytoplasm. Scale bars, 20 µm.