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. 2016 Jan 13;6(1):150197. doi: 10.1098/rsob.150197

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Expression pattern of BgN and cytoskeleton impairments in the ovaries of 0-day-old dsBgN-treated adult females. (a) BgN mRNA expression in ovaries of sixth-instar nymphs and adult females during the first gonadotrophic cycle, shows higher expression during the pre-vitellogenic period. The dashed line indicates the moult to adult, the arrow the oviposition time, and 7c the time of choriogenesis. The pre-vitellogenesis (Pre-Vg), vitellogenesis (Vg) and post-vitellogenesis (Post-Vg) stages are also indicated. Data represent copies of mRNA per 1000 copies of BgActin-5c (relative expression) and are expressed as means ± s.e.m. (n = 3). (b) Localization of BgN (NICD, magenta) in the cells placed in the germarium, in the stalk cells between the basal and sub-basal ovarian follicles, and in the FCs of the basal ovarian follicle of 0-day-old dsMock-treated adult females. (c) Ovariole from 0-day-old dsBgN-treated adult female shows the basal ovarian follicle with a spherical shape (the oocyte nucleus was labelled with the anti-Eya antibody). (d) Changes in FC planar polarity in basal ovarian follicles from 0-day-old dsBgN-treated adult females show (d′) nuclei, (d″) F-actin microfilaments and (d‴) NICD labelling (with no labelling). (e) Follicular epithelium in basal ovarian follicles from 0-day-old dsMock-treated females shows the FCs were mitotically active and the F-actin microfilaments were well distributed around the cell membranes: merged image of (e′) nuclei and (e″) F-actin microfilaments. (f) Follicular epithelium in basal ovarian follicles from 0-day-old dsBgN-treated females: merged image of (f′) nuclei and (f″) F-actin microfilaments. DAPI was used in DNA staining, and phalloidin-TRITC to stain the F-actin microfilaments. In all images, the posterior pole of the basal follicle is towards the bottom, except in (c) in which it is towards the left. Scale bars in (e,f): 20 µm; in (b,d): 50 µm and in (c): 100 µm.