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. 2016 Jan 13;6(1):150197. doi: 10.1098/rsob.150197

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — Depletion of BgDl and BgSer did not impede stalk formation. (a) mRNA expression levels of BgN, BgDl and BgSer in ovaries from 5-day-old dsBgDl + dsBgSer-treated females. Data represent values normalized against the control (reference value = 1) (n = 3). (b–e) Detail of the stalk structure connecting the basal (BOF) and the sub-basal (sBOF) ovarian follicles in 5-day-old dsMock-treated (b), dsBgDl-treated (c), dsBgSer-treated (d) and dsBgDl + dsBgSer-treated (e) females. The stalk was always formed in treated females and sometimes it is even longer than in dsMock-treated females. The F-actin microfilaments were stained with phalloidin-TRITC (green), and DNA with DAPI (blue). The posterior pole of the basal follicle is oriented towards the bottom left. Scale bars, 50 µm.