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Correlation between thermal-stability measure ∑=ΔSVH,25 /(kBNr) and denaturation temperature Tm. ΔSVH,25 is the water-entropy gain upon protein folding at 25ºC. Nr and kB are the number of residues and the Boltzmann constant, respectively. Black closed circles: group 1. Red closed circles: group 2. A mutant in group 2 is obtained by mutations including Pro→Gly.
