Skip to main content
. 2015 Oct 5;128(19):2625–2631. doi: 10.4103/0366-6999.166038

Table 1.

Primers used for amplifying the NS3, NS5A and NS5B regions

Gene fragments Primers 5’-3’sequence
NS3 Forward 1 5’-GCCGCGATGCCATCATCC-3’
Reverse 1 5’-CATTAGAGCGTCTGTTGC-3’
Forward 2 5’-CTATGGCAAAGCCATCCC-3’
Reverse 2 5’-GCCAGACTCCCTTGTACCC-3’
NS5A Forward 1 5’-GTGGAAGTGTCTCAYACG-3’
Reverse 1 5’-ATGTTYCCGCCCATCTCCTGCCG-3’
Forward 2 5’-CCCCACGCACTATGTGCC-3’
Reverse 2 5’-TARAGGGCCATYTTCTCGC-3’
NS5B Forward 1 5’-TCACAGCTCCCATGYGAGCC-3’
Reverse 1 5’-CTTYGCAGCTCGACAGGC-3’
Forward 2 5’-ACCGGGACGTGCTBAAGG-3’
Reverse 2 5’-GGGGAGCAGGTAGATGCC-3’
Forward 3 5’-CGCTGYTTTGACTCAACGG-3’
Reverse 3 5’-ATTGGCCTGGAGTGTTTAGC-3’