Table 1.
Gene fragments | Primers | 5’-3’sequence |
---|---|---|
NS3 | Forward 1 | 5’-GCCGCGATGCCATCATCC-3’ |
Reverse 1 | 5’-CATTAGAGCGTCTGTTGC-3’ | |
Forward 2 | 5’-CTATGGCAAAGCCATCCC-3’ | |
Reverse 2 | 5’-GCCAGACTCCCTTGTACCC-3’ | |
NS5A | Forward 1 | 5’-GTGGAAGTGTCTCAYACG-3’ |
Reverse 1 | 5’-ATGTTYCCGCCCATCTCCTGCCG-3’ | |
Forward 2 | 5’-CCCCACGCACTATGTGCC-3’ | |
Reverse 2 | 5’-TARAGGGCCATYTTCTCGC-3’ | |
NS5B | Forward 1 | 5’-TCACAGCTCCCATGYGAGCC-3’ |
Reverse 1 | 5’-CTTYGCAGCTCGACAGGC-3’ | |
Forward 2 | 5’-ACCGGGACGTGCTBAAGG-3’ | |
Reverse 2 | 5’-GGGGAGCAGGTAGATGCC-3’ | |
Forward 3 | 5’-CGCTGYTTTGACTCAACGG-3’ | |
Reverse 3 | 5’-ATTGGCCTGGAGTGTTTAGC-3’ |