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. 2015 Oct 5;128(19):2658–2663. doi: 10.4103/0366-6999.166037

Figure 1.

Figure 1

Effects of Sal B on MMP-9 activity and expression in TNF-α-induced human coronary artery endothelial cells. (a) Effect of Sal B on human coronary artery endothelial cells survival. Cells were incubated with Sal B (1–100 μmol/L) for 48 h and Cell Counting Kit-8 assay was performed to evaluate the cytotoxicity; (b) Cells were stimulated with 100 ng/ml TNF-α for 2–48 h. MMP-9 protein was measured by Western blot assay; (c) Cells were pretreated with Sal B (1–10 μmol/L) for 24 h and stimulated with 100 ng/ml TNF-α for 8 h. MMP-9 protein was measured by Western blot assay; (d) Secreted MMP-9 in the cell-free culture media was measured by gelatin zymogram assay. *P < 0.05, †P < 0.01 versus the untreated baseline control. ‡P < 0.05, §P < 0.01 versus the TNF-α group. Sal B: Salvianolic acid B; MMP-9: Matrix metalloproteinas-9; TNF-α: Tumor necrosis factor-α.