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. 2016 Jan 13;3(1):150424. doi: 10.1098/rsos.150424

Figure 2.

Figure 2.

In steps 2–6 of the web-based application, the user draws a best fit circle around the pupillary ruff (purple circle), collarette (blue circle) and iris (red circle). This allows the program to identify the pupillary zone (bounded by the blue and purple circles) and the ciliary zone (bounded by the blue and red circles).