Table 1.
Primer pairs used for RT-qPCR analyses (a=Forward, b=Reverse). A total of four genes were chosen for brain analysis. β-actin was chosen as the reference gene.
| short name | primer sequence | function | BLAST hit name | species | E-value |
|---|---|---|---|---|---|
| camk2g | GACGGAACTAAGGGGTCCTCa | memory, synapse, LTP | XM_006630861.1 | Lepistoseus oculatus | 0 |
| AGGTCAACCCAGGATCACAGb | GI:573886134 | ||||
| jun | GATTCGACGTTCACGGTTTTa | memory, synapse, LTP | JN257262.1 GI: 357595814 | Carassius auratus | 0.0 |
| TGTGGTTGACGCATTTCATTb | |||||
| bdnf | GGTCATCACTCTTCCCACCTa | learning behaviour, IEG | XM_010793251.1 | Notothenia coniceps | 7.0×10−66 |
| AACCATGCAATTTCCACCATb | GI: 736296403 | ||||
| egr-1 | ACCTACTCCAGTGCCAGCTCa | learning behaviour, IEG | JN230914.1 GI: 389566557 | Conger conger | 0 |
| GAACAGGTAGTCGGGGATCAb | |||||
| β-actin | CAGCCTTCCTTCCTGGGTa | housekeeping gene | DQ286836.1 GI: 82798415 | Anguilla anguilla | 0 |
| AGTATTTGCGCTCGGGTGb |