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. 2016 Jan 18;4:e1474. doi: 10.7717/peerj.1474

Table 2. PCR primers and conditions used to obtain cpDNA sequence data for Astragalus edulis.

cpDNA region Forward primer Reverse primer Denaturation Temperature/Time Annealing Temperature/Time Extension Temperature/Time Cycles
trnG-trnS 3′trnGUUC trnSGCU 95°C/30″ 62°C/30″ 72°/1′30″ 35
trnC-rpoB trnCGCAR rpoB 95°C/30″ 55°C/30″ 72°/1′30″ 35
tabC-tabF trnLUAA5′ trnFGAA 95°C/30″ 52°C/30″ 72°/2′30″ 35