Table 2. PCR primers and conditions used to obtain cpDNA sequence data for Astragalus edulis.
cpDNA region | Forward primer | Reverse primer | Denaturation Temperature/Time | Annealing Temperature/Time | Extension Temperature/Time | Cycles |
---|---|---|---|---|---|---|
trnG-trnS | 3′trnGUUC | trnSGCU | 95°C/30″ | 62°C/30″ | 72°/1′30″ | 35 |
trnC-rpoB | trnCGCAR | rpoB | 95°C/30″ | 55°C/30″ | 72°/1′30″ | 35 |
tabC-tabF | trnLUAA5′ | trnFGAA | 95°C/30″ | 52°C/30″ | 72°/2′30″ | 35 |