Figure 3.

(a) Baseline reactive oxygen species differences between Wistar and GK SMCs in NG (5 mM). Hydrogen peroxide was measured by Amplex Red (n = 3), and superoxide was detected as a percentage of total mitochondria using MitoTracker and MitoSOX fluorescence on live cells with flow cytometry (n = 3). Representative flow cytometry figures of Wistar SMCs (left) and GK SMCs (right) are presented. (b) Mitochondrial antioxidant status was measured with Western blot, 15–30 μg protein on an SDS-page gel, and data are normalized to β-actin and expressed as mean fold change from Wistar SMCs + SEM. Significance measured by Student's t-test, ^∗ p < 0.05.