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. 2016 Jan 11;2016:8524267. doi: 10.1155/2016/8524267

Figure 5.

Figure 5

(a) Time course comparisons of AMPK (n = 3), pAMPK (n = 4), peNOS (n = 3), PGC-1α (n = 3), and VDAC1 (n = 3), and mitochondrial complex expression in Wistar and GK SMCs, passages 8–10. (b) Superoxide (flow cytometry, n = 3) and antioxidant status (Western blot, MnSOD n = 4, UCP3 n = 3) differences between Wistar and GK SMCs in NG (5 mM) or HG (25 mM). Protein expression was measured using Western blot, 15–30 μg protein on an SDS-page gel, and data are normalized to β-actin and expressed as mean fold change from NG + SEM. (c) Assessment of mitochondrial morphology (n = 3) during HG (25 mM) time course. Representative photographs of fixed SMCs using TOM 20 (green) and nitrotyrosine (red) are shown. (d) Time course mitochondrial dynamic (n = 4) and autophagy (n = 3) differences in Wistar and GK SMCs, passages 8–10. Protein expression was measured using Western blot, 15–30 μg protein on an SDS-page gel, and data are normalized to β-actin and expressed as mean fold change from NG + SEM. Significance measured by one-way ANOVA, p < 0.05 (∗ compared to NG, ‡ compared to 1-hour HG), † p < 0.1.