Table 1.
Mitochondrial respiration and ATP concentrations as measured with O2k from Oroboros and ATP fluorometric assay. Respiration baseline comparisons are between Wistar (n = 6) and GK (n = 3) SMCs in NG (5 mM), Wistar, or GK SMCs, passages 8–10. ATP concentrations (µM/1 × 106 cells) are between Wistar (n = 8) and GK (n = 8). Respiration was measured as oxygen flux (pmol second−1 million cells−1) ± standard error of the mean (SEM). ∗ p < 0.05 as measured by Student's t-test.
| Respiration states | Wistar | GK |
|---|---|---|
| S2 (PMG) | 18.35 ± 3.97 | 29.79 ± 5.38 |
| S3 (PMG) | 75.99 ± 19.04 | 153.88 ± 23.82∗ |
| S3 (PMG/S) | 187.82 ± 40.96 | 241.30 ± 36.46 |
| S4 (PMG/S) | 54.88 ± 8.33 | 52.52 ± 4.56 |
| Uncoupled | 262.98 ± 46.06 | 375.15 ± 20.63 |
| RCR | 3.35 ± 0.29 | 4.56 ± 0.45∗ |
| ATP | 8.64 ± 1.91 | 7.14 ± 1.08 |