Table 1.
Primers and conditions used for RT-PCR.
| Gene | Forward primer (5′-3′), reverse primer (5′-3′) |
T-An. (°C) | Gene | Forward primer (5′-3′), reverse primer (5′-3′) |
T-An. (°C) |
|---|---|---|---|---|---|
| Mfn-1 | ATGGCAGAAACGGTATCTCCA CTCGGATGCTATTCGATCAAGTT |
60.5 | Drp-1 | CAGGAATTGTTACGGTTCCCTAA CCTGAATTAACTTGTCCCGTGA |
60 |
|
| |||||
| Mfn-2 | CTGGGGACCGGATCTTCTTC CTGCCTCTCGAAATTCTGAAACT |
61 | Fis-1 | TGTCCAAGAGCACGCAATTTG CCTCGCACATACTTTAGAGCCTT |
62 |
|
| |||||
| Opa-1 | TGGAAAATGGTTCGAGAGTCAG CATTCCGTCTCTAGGTTAAAGCG |
60.5 | GAPDH | TGGATTTGGACGCATTGGTC TTTGCACTGGTACGTGTTGAT |
60.5 |
T-An.: annealing temperature; Mfn-1: mitofusin 1; Mfn-2: mitofusin 2; Opa-1: optic atrophy 1; Drp-1: dynamin-related protein q; Fis-1: mitochondrial fission 1 protein.