Fig. 1.

CHK1 and CHK2 bind tZ in an in vivo cytokinin binding assay. All receptors were expressed as GST fusion proteins in E. coli strain BL21 (DE) pLys. The specific binding to trans-[2-³H]zeatin was analyzed according to Romanov and Lomin (2009). Shown are biological replicates (n=3) and their SD (error bars). Expression of the different fusion proteins was confirmed by western blot (see Supplementary Fig. S1 at JXB online).