Average level of isoprene-type cytokinins in tissue of P. patens wild type and the cytokinin receptor mutants Δchk1,2,3 (triple) and Δchk1,2; Δchk1,3 and Δchk2,3 (double). Each genotype was cultured three times independently for 21 d, sampled at three independent time points, and measured by UHPLC-MS/MS (n=9–24). Data present mean values with 95% confidence intervals. Cytokinin content in the mutants was compared with that in the wild type by independent samples Kruskal–Wallis test (confidence interval 95%, significance level 0.5); no significant changes compared in all samples with the wild type. Values are given in pmol g–1 DW. (A) Sum of all measured cytokinins. (B–S) Levels of each individual cytokinin; columns are the same as indicated in (A). tZ, trans-zeatin; tZOG, trans-zeatin O-glucoside; tZR, trans-zeatin riboside; tZRMP, trans-zeatin riboside-5′-monophosphate; tZROG, trans-zeatin riboside O-glucoside cZ, cis-zeatin; cZOG, cis-zeatin O-glucoside; cZR, cis-zeatin riboside; cZRMP, cis-zeatin riboside-5′-monophosphate; cZROG, cis-zeatin riboside O-glucoside; DHZ, dihydrozeatin; DHZOG, dihydrozeatin O-glucoside; DHZR, dihydrozeatin riboside; DHZRMP, dihydrozeatin riboside-5′-monophosphate; DHZROG, dihydrozeatin riboside O-glucoside; iP, N
6-isopentenyladenine; iPR, N
6-isopentenyladenosine; iPRMP, N
6-isopentenyladenosine-5′-monophosphate. udl, under the detection limit.