Fig. 3.

Relevant sections of GC-MS traces, shown as extracted ion chromatograms for marker ion 204, for WT VvGuaS (black), and mutant enzymes T414S (pink), V530M (blue), and T414S/V530M (brown). Major peaks corresponding to specific sesquiterpenes are indicated with black arrows. The vertical dotted line delineates the discontinuous chromatogram. Above are the structures of four major products of WT VvGuaS, comprising (from left to right) α-guaiene, γ-gurjunene, epiglobulol, α-bulnesene, and pogostol. Below are four of the major products of T414S/V530M, and also VvPNSeInt, comprising selina-4,11-diene, α-selinene, epi- α-selinene, and intermedeol.