Table 1.
Volatile metabolic products of VvGuaS expressed in N. benthamiana
| TIC% | |||||||
|---|---|---|---|---|---|---|---|
| Compound | RI | Ref RIb | WT | T414S | V530M | T414S/V530M | VvPNSeIntc |
| α-copaenea | 1373 | 1372–1376 | 2 | 3 | 1 | 1 | - |
| α-gurjunene | 1407 | 1402–1411 | 1 | 1 | 0 | 0 | - |
| α-guaiene a | 1437 | 1428–1437 | 44 | 41 | 24 | 5 | 3.5 |
| Unknown | 1440 | n/a | 0 | 0 | 0 | 1 | - |
| γ-gurjunene | 1478 | 1479 | 4 | 3 | trace | 0 | - |
| Selina-4(14),11-diene | 1482 | 1472–1488 | 0 | 4 | 24 | 40 | 34 |
| Epiglobulol | 1488 | 1463–1497 | 6 | 12 | 2 | 2 | - |
| α-selinene | 1492 | 1478–1497 | 0 | 7 | 16 | 23 | 14 |
| Unknown | 1498 | n/a | 5 | 5 | 4 | 4 | - |
| α-bulnesene a | 1506 | 1500–1515 | 35 | 18 | 12 | 0 | - |
| Epi-α-selinene | 1517 | 1518 | 1 | 4 | 8 | 12 | 15 |
| δ-cadinene a | 1524 | 1519–1530 | 0 | 0 | 3 | 2 | - |
| Pogostol | 1653 | 1637–1656 | 3 | 2 | 0 | 0 | - |
| Intermedeol | 1660 | 1654–1667 | 0 | 2 | 8 | 9 | 30 |
TIC percentages are semi-quantitative because they depend on each compound’s affinity for the SPME fibre. Compound identities reported based on comparison to authentic standards (a) or putatively annotated based on mass spectrum similarity (>90% as calculated by Shimadzu GCMS Solution 2.50 software against compounds in NIST08 and Wiley08 libraries) and RI within the range of reported references, except for epi-α-selinene and intermedeol, which are based on RI alone. b Ranges of reported retention indices for DB5 and similar columns as compiled in the NIST Standard Reference Database 69: NIST Chemistry WebBook. c Products previously reported for VvPNSeInt shown for comparison (Martin et al., 2010).