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. 2015 Nov 23;67(3):873–891. doi: 10.1093/jxb/erv502

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Effect of Cl^– nutrition on photosynthesis, cell division, and growth. In (A–D), treatments consisted of the application of the basal nutrient solution (BS) alone or supplemented with 5mM chloride (CL), 5mM nitrate (N), or the sulphate+phosphate (SP) salt mixture containing the same cationic balance as in the CL and N treatments. (A) Effect on net photosynthetic rate (A _N) measured in fully expanded, photosynthetically active leaves from tobacco plants between 53 d and 62 d after sowing (DAS). (B) Effect on cell division rate, quantified as the number of epidermal cells per leaf (abaxial side). (C, D) Effect on total (shoot and roots) FW and DW at the end of the experiment. (E, F) Effect of increasing concentrations of Cl^– (CL) or sulphate+phosphate (SP) treatments, maintaining the same cationic balance, on total (shoot and roots) FW and DW, respectively. A non-linear scale is used on the horizontal axis for dose–response experiments (E and F). Mean values ± SE, n=6. Levels of significance: *P≤0.05; **P≤0.01. ‘Homogeneous group’ statistics were calculated through ANOVA (A–F) and MANOVA (A, E, F) tests.