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. 2015 Nov 23;67(3):873–891. doi: 10.1093/jxb/erv502

Fig. 3.

Fig. 3.

Effect of Cl– nutrition on leaf cell size. In (A–G), treatments consisted of the application of the basal nutrient solution (BS) alone or supplemented with 5mM chloride (CL), 5mM nitrate (N), or the sulphate + phosphate (SP) salt mixture containing the same cationic balance as in the CL and N treatments. In (H), treatments consisted of increasing concentrations of Cl– (CL) or sulphate+phosphate (SP), maintaining the same cationic balance. (A, H) Effect on epidermal cell size. (B) Effect on palisade mesophyll cell size. (C) Effect on spongy mesophyll cell size. (D) Effect on stomatal size (combined surface area of the two guard cells and the pore space between). (E) Effect on trichome length. (F) Effect on leaf thickness. (G) Effect of the treatments BS, N, and CL on epidermal cell size observed in microscopy images obtained from abaxial leaf epidermal impressions. Epidermal and stomatal size values were obtained from abaxial epidermal imprints; mesophyll cells size and leaf thickness values were obtained from transversal sections; and trichome length was directly measured from epidermal peel preparations. A non-linear scale is used on the horizontal axis for the dose–response experiment (H). Mean values ± SE, n=6. Levels of significance: ***P≤0.001; and ‘homogeneous group’ statistics were calculated through ANOVA (A–F, H) and MANOVA (H) tests.