Skip to main content
. 2015 Aug 19;138(1):206–216. doi: 10.1002/ijc.29680

Figure 2.

Figure 2

Enrichment for nonhaematopoietic cells. Cultured malignant cells were added to 4 ml of whole blood and cells were incubated with tetrameric antibody complexes against CD45 and dextran‐coated magnetic particles for 1 hr and placed in an EasySep Big Easy magnet. Cells not attracted to the magnet were recovered. The number of leukocytes was counted with a haemocytometer prior to and after depletion. Erythrocytes were removed by lysis and platelets by centrifugation at 250 g.