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. 2014 May 27;66(6):1492–1503. doi: 10.1002/art.38392

Figure 5.

Figure 5

ES‐62 desensitizes IL‐17–mediated production of IL‐6 by synovial fibroblasts. A, CD90.2, CD54, and CD106 expression (black lines) by synovial fibroblasts from mice with CIA (gray lines represent isotype controls). Fibroblasts were cultured ex vivo for 7 days prior to in vitro stimulation. B, IL‐6 production by synovial fibroblasts from PBS‐treated mice with CIA, in response to recombinant IL‐17 (rIL‐17) or rIL‐22 (10 ng/ml). Three independent cultures of cells obtained by pooling mice (n = 7) within each group were performed. Values are the mean ± SEM (calculated using the mean result from the triplicate experiments). C, IL‐6 production over 24 hours by synovial fibroblast explant cultures (106/ml) from individual PBS‐treated mice with CIA or ES‐62–treated mice with CIA, in response to fresh medium, rIL‐17 (10 ng/ml), or rIL‐22 (10 ng/ml). For PBS‐treated mice, n = 12, 11, and 12 for fresh medium, rIL‐17, and rIL‐22, respectively; for ES‐62–treated mice, n = 14, 13, and 14, respectively. Each symbol represents the mean value from triplicate determinations (by enzyme‐linked immunosorbent assay) in an individual mouse; bars show the mean. ∗ = P < 0.05; ∗∗∗ = P < 0.001. See Figure 1 for other definitions.